Hossein Daghigh Kia; Zahra Blouki
Volume 19, Issue 3 , November 2017, , Pages 727-738
Abstract
The aim of this study was to investigate the antioxidant effect of coenzyme Q10 on ram semen quality after the freezing-thawing process. Five Ghezel rams were used for sperm collection twice a week in five replicates. This experiment consisted of 5 treatments, coenzyme Q10 at four levels (0.5, 1, 2 and ...
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The aim of this study was to investigate the antioxidant effect of coenzyme Q10 on ram semen quality after the freezing-thawing process. Five Ghezel rams were used for sperm collection twice a week in five replicates. This experiment consisted of 5 treatments, coenzyme Q10 at four levels (0.5, 1, 2 and 2.5 μmol) and control group (without antioxidant). In this study, motility, viability, membrane integrity, abnormality parameters of sperm, malondialdehyde, total antioxidant capacity, glutathione peroxidase and superoxide dismutase activity were measured following freeze-thawing. The results showed that the total motility in samples with 0.5 and 1 μM of coenzyme Q10 antioxidant was significantly higher than the control group (p< 0.05). Samples with 0.5 μM Coenzyme Q10 had the lowest percentage of abnormal sperm and lipid peroxidation level compared to the control group (p< 0.05). Viability, plasma membrane integrity and total motility were better in all treated groups compared to the control group (p< 0.05). The VCL, VSL and VAP parameters were improved in groups receiving 0.5, 1 and 2 μM coenzyme Q10 compared with the other groups (p< 0.05). The group receiving 2.5 μM coenzyme Q10 had the best impact on the superoxide dismutase activity than other groups. The results of this study showed that adding 0.5 and 1 μM Coenzyme Q10 improved some of the sperm parameters after the freezing-cracking process.
Hossein Daghigh Kia; Fatemeh Zare Ghaleh Jigh; Abouzar Najafi; Hossein Vaseghi Dodran
Volume 18, Issue 4 , December 2016, , Pages 831-840
Abstract
In order to improve the quality of ram semen after freezing-thawing process, different levels of ethanol extract of Bilberry plants were investigated. In this study five rams were used for semen collection twice a week by an artificial vagina. In order to eliminate the effects of the individual, the ...
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In order to improve the quality of ram semen after freezing-thawing process, different levels of ethanol extract of Bilberry plants were investigated. In this study five rams were used for semen collection twice a week by an artificial vagina. In order to eliminate the effects of the individual, the semen samples were pooled. Different levels of Bilberry extract (0, 100, 150, 200 μl/ml) were added to the Tris- Egg yolk based extender. Following cooling and freezing of semen samples, they were stored in liquid nitrogen until evaluation. After freezing-thawing, motility parameters were evaluated using the Computer-assisted sperm analysis system (CASA), the viability of sperms by Eosin-Nigrosin staining, membrane integrity using the hypo-osmotic swelling test, sperm abnormality by Hancock solution and lipid peroxidation by measuring malondialdehyde concentration. The results showed that the addition of 150 μl of Bilberry extract improved sperm parameters following freezing-thawing process compared to the control group (P< 0.05). According to the results of this study, Bilberry extract can be used as a natural antioxidant, with high performance, inexpensive and available in ram sperm diluent.